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The Mouse Imaging Centre has one OPT device:

The system was custom built at MICe by Michael Wong and Jun Dazai. Design and implementation details can be found in their 2013 Plos ONE paper.

OPT Sample Preparation

OPT Sample Imaging

  • General imaging instructions are as follows:
  1. Please see above OPT Sample Preparation section for instructions on how to prepare your sample for imaging
  2. Once your sample is embedded in agar and has been sufficiently cleared in BABB (ie. agar and sample are transparent), it is ready for imaging 
  3. Remove the agar-embedded sample from the vial and place it on a piece of clean gauze - pat the agar with gauze to remove excess BABB
    • wrap the agar in the gauze and allow to sit for at least 30 minutes
  4. Take a clean metal chuck (wipe surface with methanol) and apply a small amount of gel super glue (blue container) - enough for a thin layer - over the top of the chuck. Allow to sit for 5 minutes so the glue becomes tacky.
  5. Place agar-embedded sample on to the top of the metal chuck - allow to set for at least an hour
    • you can prepare mutliple samples/chucks at one time
  6. While you wait, turn on the following OPT components
    • UV light source (this will take several minutes to warm up)
    • microscope controller
    • CCD camera
    • white light source
  7. On OPT computer
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